12/9/2023 0 Comments Flowjo ed reddit![]() This implies that B-cells are associated with ENL pathology. We found increased antigen experienced and activated B-cells in untreated ENL patients compared to those without the reaction (LL patients). In the present study we described the role of B-cell subsets in ENL reaction and compared with non reactional LL patient controls before, during and after corticosteroids treatment. However, the contribution of B-cells in ENL reactions has never been addressed. Immune-complexes and T-cells are suggested as the aetiology of ENL. Type 2 or Erythema nodosum leprosum (ENL) is an immune-mediated inflammatory complication of leprosy which occurs in lepromatous and borderline lepromatous leprosy patients. There are two types of leprosy reactions, type 1 and type 2 reactions. Some leprosy patients develop reactions which cause a significant morbidity and mortality in leprosy patients. The mechanism by which inflammatory reactions like ENL affecting these memory cells and contributing to the disease pathology is an interesting area to be explored for and could lead to the development of novel and highly efficacious drug for ENL treatment. The finding of increased percentage of activated memory B-cells in untreated patients with ENL reactions suggests the association of these cells with the ENL pathology. However, the generation and differential function of these memory B-cells need further investigation. Conversely, the increased production of activated memory B-cells in ENL patients could imply the scale up of immune activation through antigen presentation to T-cells. It appears that the lower frequency of tissue-like memory B-cells in untreated ENL could promote the B-cell/T-cell interaction in these patients through downregulation of inhibitory molecules unlike in LL patients. On the other hand, the percentage of tissue-like memory B-cells was considerably low in untreated ENL patients compared to LL controls. Interestingly, while the percentage of naive B-cells was significantly lower in untreated ENL patients than in LL patient controls, the percentage of activated memory B-cells was significantly higher in these untreated ENL patients than in LL controls. Total B-cells, mature B-cells and resting memory B-cells were not significantly different between patients with ENL reactions and LL controls before treatment. The kinetics of B-cells in patients with ENL before, during and after Prednisolone treatment of ENL was compared with LL patient controls as well as within ENL group. Peripheral blood mononuclear cells (PBMCs) were isolated and used for immunophenotyping of B- cell subsets by flow cytometry. Peripheral blood samples were obtained before, during and after treatment from each patient. A case-control study design was used to recruit 30 untreated patients with ENL and 30 non-reactional lepromatous leprosy (LL) patient controls at ALERT Hospital, Ethiopia. Therefore, it would be interesting to examine the contribution of B-cells in the pathogenesis of ENL. Although Erythema Nodosum Leprosum (ENL) is an inflammatory complication of leprosy, the role of B- cell subsets has never been studied in this patient group. B-cells, in addition to antibody secretion, have emerged increasingly as effector and immunoregulatory cells in several chronic inflammatory diseases.
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